The main objective of this study is to describe the effectiveness of learning based on 5E instructional model to improve science process skills and students’ concept mastery. The science process skills is important for students as it is the foundation in enhancing the mastery of concepts and thinking skills needed in the 21^st century. The design of this study was one group pretest-posttest design. The result of the study shows that (1) the implementation of learning in both of classes, IVA and IVB, show that the percentage of learning implementation is increasing which indicates the better quality of learning. (2) the percentage of students' science process skills test result on the aspect of observing, formulating hypotheses, determining, interpreting data and communicating were increased and (3) the percentage of students who know the concept was increased and there is a decrease in the number of students who don’t know the concept and misconception.