Surface chemistry lecture is one of lectures that required laboratory activity in teaching and learning process. Laboratory activity is a chance for chemistry educator to train students’ hands on minds on activity. Worksheet as a guide for student to conduct experiment in the laboratory usually act as recipe. This kind of worksheet is impossible to help students use their higher order thinking. A worksheet has to be set with activity which is help students to arrange their own experiments. For this reason, this research is focused on how to train students higher order thinking skills in laboratory activity by developing laboratory activity worksheet on surface chemistry lecture. To ensure that the practicum guide already contains aspects of the higher order thinking skill, it requires theoretical and empirical validation. From the results of data analysis can be said that the developed worksheet worth to use. The worksheet is worthy of theoretical feasibility and empirical feasibility. This conclusion is based on the findings:1) Assessment of the validator of theoretical feasibility aspects in the category is very feasible with an assessment range of 95.24% to 97.92%. 2) students’ higher thinking skill from N Gain values ranges from 0.50 (enough) to 1.00 (high) so it can be concluded that the laboratory activity worksheet on surface chemistry lecture is empirical in terms of worth. The empirical feasibility is also supported by the responses of the students in very reasonable categories.It is expected that the laboratory activity worksheet on surface chemistry lecture can train students high order thinking skills for students who program surface chemistry lecture.