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This study is to obtain the role of yacon tubers through storage and boiling variations as prebiotics and sinbiotics against Salmonella typhimurium pathogenic bacteria (in-vivo). There are 80 of Rattus norvegicus that were divided into 10 groups: five of them by yacon tubers prebiotic supplements and five other feed by yacon tubers sinbiotics with Bifidobacterium longum Reuter ATCC 15707, infected on days 22 and 23. Salmonella typhimurium IFO 12529 infections is on days 29 and 30. Total length of yacon tubers supplements is 40 days. There are four kinds of treatment for yacon tubers, namely, T1 (without storage and boiling), T2 (without storage, 60 minute boiling), T3 (14 day storage, no boiling), T4 (14 day storage, 60 minute boiling). The results of the study: 1) The use of prebiotic yacon tubers inhibits growth or decreases the amount of Salmonella, and it is more effective to use yacon tubers sinbiotics along with Bifidobacterium longum, 2) Among the yacon tubers treatment supplements, the T1 is the most effective treatment for decreasing the amount of Salmonella, with or without Bifidobacterium longum.

Yacon Tubers, Prebiotics, Sinbiotics, Salmonella typhimurium IFO 12529, Rattus norvegicus