Soybeans are national food crop commodity with a high level of consumption per year. Efforts to increase soybean production are accomplished through fertilizer application. The utilization of chemical fertilizers, however, can negatively impact the environment. One alternative to substitute for fertilizer is to provide superior microbes that are beneficial to the plants. This study aimed to determine how the application of rhizobacteria and indigenous microorganism in cow rumen affects the growth of soybean plants. This study method included isolate rejuvenation, identification and selection for soybean plant application using one factor of a completely randomized design method consisting of five treatment levels. The results indicated that the superior isolates, AJ8, had the highest potential utilization as Indole Acetic Acid hormone producer, while MTA1 isolates were phosphate solvent isolates. The identification results using Microbact showed that AJ8 isolates had 99% similarity with Acinetobacter baumanii, while MTA1 isolates were identified as Propionibacterium granulosum. Application of Acinetobacter baumanii AJ8 and Propionibacterium granulosum MTA1 in soybean plants had a significant effect on the number of lateral roots compared to the negative control and NPK fertilizer utilization. The potential use of the superior bacteria discovered still needs further study until the biological fertilizer formulations formed can reduce the utilization of chemical fertilizers.