The purpose of this study was to determine cholesterol concentration by developing the performance of optical sensors. The use of this optical sensor is equipped with optical fiber bundle to guide the wave in the hope of not reducing the intensity of the wave source. This sensor is more simple and inexpensive to use to determine the concentration of a material. The research method for determining cholesterol levels can be determined through the character of the laser beam to the material through a fiber optic sensor. Optical fiber sensor method is simpler and cheaper to use to determine the concentration of a material. Based on this description, a research on fiber optic sensors will be developed as an instrument model to determine cholesterol concentration using a fiber optic bundle type sensor. The method used is the modulation of laser beam intensity. A beam of light that enters the optical fiber of the receiver and is processed by an optical detector into an electrical signal that will be displayed on the computer. The experimental results will obtain a linear relationship between the sensor output peak voltage as a function of variations in cholesterol concentration and parameters and sensor performance will be measured including sensitivity and linearity. The results showed that maximum voltage detection showed a linear decrease with increasing concentration with a sensitivity of 0.15 mV / ppm and linearity of more than 97%. high sensitivity, simplicity of design, and low cost of promoting the developed sensor results in good performance detecting cholesterol concentration.