Amylase produced by bacteria or bacteria amylase is an enzyme that can hydrolyze starch to sugar. Bacteria can grow well under normal environmental conditions, but some bacteria can grow in extreme environmental conditions, such as thermophile bacteria. Thermophile bacteria are bacteria that can live at temperatures above 45ºC. Thermophile bacteria can produce various enzymes, such as proteases, lipases, cellulases, and amylases. Amylase has a wide application in the industrial field because it is stable, has high enzyme activity at various parameters. Amylase is important due to their commercial applications in starch liquefaction, paper, textile fabrics, brewing industry, sugar induction by the production of sugar syrups, pharmaceuticals, and preparing cold water dispersible laundry. Amylase is one of the three largest groups of industrial enzymes and accounts for approximately 65% of the world enzyme.

 

Amylase derived from the thermophilel bacteria is widely used in various industries because it is stable to high temperature and pH, such as bacteria derived from hot springs Pariangan. Isolation and screening of amylase-producing bacteria originating from Pariangan hot spring, Tanah Datar regency, West Sumatera province have been successfully performed. There were 4 isolates of amylase-producing bacteria and 1 bacterium isolate with the highest activity, ie isolate S2.3. The characterization and analysis of the biochemical properties of isolate S2.3 are not known. The objective of the study was to investigate the morphology and biochemical properties of isolate S2.3.

 

The method used is characterization of isolate S2.3 (color, shape, edges, and elevation), Gram staining (morphology and spores), biochemical properties of isolate s2.3 (TSIA, Catalase, Oxidase, Indole, Urea Citrate, Lactose , Glucose, Sucrose, Mannitol, MR, VP, OF, Arabinose, Nitrate, and Gelatin.

 

Table 1. The result of a test of biochemical properties

No.

Test of biochemical properties

 

Result

1

TSIA

Red/yellow

2

Catalase

+

3

Oxidase

-

4

Indole

-

5

Urea

+

6

Citrate

-

7

Lactose

-

8

Glucose

+

9

Sucrose

+

10

Mannitol

+

11

MR

+

12

VP

-

14

OF

-

15

Arabinose

-

16

Nitrate

+

17

Gelatine

+

18

Xylose

The result of this study showed that the bacteria isolate S2.3 had white colonies, rounded shape, flat surface, and flat elevation. Isolate bacterium s2.3 is a Gram-positive bacterium and has spores. The result of the test of biochemical properties (Table 1) showed that bacteriua isolate of S2.3 was classified into Bacillus sp.